Isolation and Identification of Stilbenes in Two Varieties of Polygonum c uspidatum

作者：Bret C. Vastano, Yong Chen, Nanqun Zhu, Chi‐Tang Ho, Zhengyi Zhou, Robert T. Rosen · 发表于：Journal of Agricultural and Food Chemistry · 年份：2000 · DOI：10.1021/jf9909196 · 被引用次数：252 · 研究领域：Sirtuins and Resveratrol in Medicine、Bioactive natural compounds、Plant Gene Expression Analysis

The roots of two varieties of Polygonum cuspidatum (Hu Zhang and Mexican Bamboo) were analyzed for resveratrol and analogues. The roots of each variety were dried and ground into a powder. The powdered roots were then extracted with methanol and ethyl acetate. The ethyl acetate fraction of the Mexican Bamboo was then subjected to fractionation and purification using silica gel column chromatography and semipreparative HPLC. In addition to resveratrol (3,5,4'-trihydroxystilbene), three stilbene glucosides were identified by (1)H NMR, (13)C NMR, and MS. The stilbene glucosides were shown to be a piceatannol glucoside (3,5,3', 4'-tetrahydroxystilbene 4'-O-beta-D-glucopyranoside), resveratroloside (3,5,4'-trihydroxystilbene 4'-O-beta-D-glucopyranoside), and piceid (3,5,4'-trihydroxystilbene 3-O-beta-D-glucopyranoside). The levels of the piceatannol glucoside and piceid were twice as high in the Mexican Bamboo as compared to the Hu Zhang.