N6-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO

作者：Gui-Fang Jia, Ye Fu, Xu Zhao, Qing Dai, G. Zheng, Ying Yang, C. Yi, Tomas Lindahl, Tao Pan, Yun-Gui Yang, Chuan He · 发表于：Nature Chemical Biology · 年份：2011 · DOI：10.1038/nchembio.687 · 被引用次数：752 · 研究领域：Biology、Medicine

We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N6-methyladenosine (m6A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m6A in mRNA, whereas overexpression of FTO resulted in a decreased level of m6A in human cells. We further show that FTO partially colocalizes with nuclear speckles, supporting m6A in nuclear RNA as a physiological substrate of FTO.