High-Resolution N6-Methyladenosine (m6A) Map Using Photo-Crosslinking-Assisted m6A Sequencing

作者：Kai Chen, Zhike Lu, Xiao Wang, Ye Fu, Guan-Zheng Luo, Nian Liu, Dali Han, D. Dominissini, Qing Dai, T. Pan, Chuan He · 发表于：Angewandte Chemie · 年份：2014 · DOI：10.1002/anie.201410647 · 被引用次数：386 · 研究领域：Chemistry、Medicine

N6-methyladenosine (m6A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m6A modification remain limited. We present here a photo-crosslinking-assisted m6A sequencing strategy (PA-m6A-seq) to more accurately define sites with m6A modification. Using this strategy, we obtained a high-resolution map of m6A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m6A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA-binding proteins.