PKC-iota drives EGFR-TKI resistance in EGFR-mutated NSCLC by phosphorylating FASN to reprogram lipid metabolism

作者：Lin Ma, Yingwen Han, Lu Xu, Qingyuan Sun, Siyue Song, Huizhen Yang, Xinran Chen, W. Xie, Yumei Chen, Liu Liu · 发表于：Translational Lung Cancer Research · 年份：2026 · DOI：10.21037/tlcr-2025-aw-1260 · 研究领域：Medicine

Background Non-small cell lung cancer (NSCLC) remains a significant challenge to global public health issues. However, epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance inevitably occurs in treating EGFR-mutant NSCLC, and the underlying metabolic mechanisms remain unclear. The objective of our study is to explore the regulatory mechanisms of the atypical protein kinase C-iota (PKC-iota) in reprogramming lipid metabolism and whether this regulation contributes to the EGFR-TKI resistance in EGFR-mutant NSCLC. Methods We use mass spectrometry (MS), co-immunoprecipitation, proximity ligation assays and molecular docking to investigate the interaction of PKC-iota and fatty acid synthase (FASN). Subsequently, Western blot assay, MS, lipid staining, membrane fluidity assay, and membrane proteins assay were performed to investigate how PKC-iota regulated the lipid metabolism by FASN. We established four types of transiently transfected H1975 and PC9 cell lines. These models were then employed in a series of assays—including Cell Counting Kit-8 (CCK-8), flow cytometry, cell counting, and colony formation—to evaluate changes in cell proliferation and EGFR-TKIs sensitivity. Four types of stably transfected H1975 cell lines were inoculated into female BALB/c nude mice, then the tumorigenicity and osimertinib sensitivity of the cell groups were analyzed. Finally, we collected 45 tumor samples of EGFR-mutated NSCLC patients to examine the clinical significanc...