Comprehensive characterization of a microfluidic platform for DEP manipulation and bio-impedance detection using multi-sized polystyrene microbeads

作者：Sameh Sherif, Y. Ghallab, Yehea Ismail · 发表于：Microfluidics and Nanofluidics · 年份：2025 · DOI：10.1007/s10404-024-02785-1 · 被引用次数：3

Dielectrophoresis (DEP) manipulation combined with micro-electric impedance spectroscopy (µEIS) presents a sophisticated approach for cellular analysis and dielectric characterization. While conventional cell analysis techniques rely on complex labeling methods with inherent limitations, integrating DEP and µEIS offers non-invasive, label-free cellular characterization with enhanced sensitivity. This study presents an innovative dual-mode DEP platform incorporating both levitation (LEVDEP) and rotational (ROTDEP) forces, integrated with high-precision impedance measurement capabilities on one chip, enabling simultaneous Cell controlling and manipulation and dielectric signature extraction within a single microfluidic device. The fabricated and developed microfluidic platform demonstrated exceptional particle discrimination through the dual mode, with distinct responses for both particle populations. Under FlEV.DEP10.4μm\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$F_{lEV.DEP}^{10.4 \mu m}$$\end{document} 2.01 MHz showed a 63.4% magnitude increase, while FlEV.DEP24.9μm\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$F_...