Virus Structures and Dynamics by Magic-Angle-Spinning NMR

作者：Gal Porat-Dahlerbruch, A. Goldbourt, T. Polenova · 发表于：Annual Review of Virology · 年份：2021 · DOI：10.1146/annurev-virology-011921-064653 · 被引用次数：23 · 研究领域：Medicine

Techniques for atomic-resolution structural biology evolved during the last several decades. Breakthroughs in instrumentation, sample preparation, and data analysis that occurred in the past decade have enabled characterization of viruses with unprecedented level of detail. Here we review the recent advances in magic-angle spinning (MAS) NMR spectroscopy for structural analysis of viruses and viral assemblies. MAS NMR is a powerful method that yields information on three-dimensional structures and dynamics in a broad range of experimental conditions. After a brief introduction, we discuss recent structural and functional studies of several viruses investigated with atomic resolution at various levels of structural organization, from individual domains of a membrane protein reconstituted into lipid bilayers to virus-like particles and intact viruses. We present examples showing the unique information revealed by MAS NMR about drug binding, conduction mechanisms, interactions with cellular host factors, and DNA packaging in biologically relevant environments, inaccessible by other methods.