Toward a national eDNA strategy for the United States

作者：R. Kelly, D. Lodge, Kai N. Lee, S. Theroux, Adam J. Sepulveda, C. Scholin, J. Craine, Elizabeth Andruszkiewicz Allan, K. Nichols, K. Parsons, K. Goodwin, Zachary Gold, F. Chavez, R. Noble, C. Abbott, M. Baerwald, A. Naaum, P. Thielen, A. Simons, C. Jerde, J. Duda, Margaret E. Hunter, John A. Hagan, R. Meyer, J. Steele, M. Stoeckle, Holly M. Bik, Christopher P. Meyer, E. Stein, K. James, Austen C. Thomas, Elif Demir‐Hilton, M. Timmers, J. Griffith, M. Weise, S. Weisberg · 发表于：Environmental DNA · 年份：2023 · DOI：10.1002/edn3.432 · 被引用次数：85

analyzed quickly and cheaply. Widespread methods of analyzing eDNA currently include single-species assays using quantitative PCR (qPCR) or digital PCR (dPCR), and multi-species amplicon sequencing (metabarcoding); qPCR studies have become common over the past decade to track the movement, abundance, and interactions of species over increasingly broad geographic scales (Beng & Corlett, 2020; Miya, 2022 ), and eDNA metabarcoding work has begun to generate multispecies and community-level views of the same phenomena.