Models of high-grade serous carcinoma of tubo-ovarian origin

作者：Achuth Padmanabhan, S. Dwivedi, Melissa A. Merritt, Priyanka Verma, R. Buckanovich, Jessica D. Lang, Hannah L Dimmick, K. Nephew, Ronny Drapkin, Lindsay W Brubaker, Benjamin G. Bitler · 发表于：Oncology Reviews · 年份：2026 · DOI：10.3389/or.2026.1849834 · 研究领域：Medicine

Biological models aim to recapitulate the molecular and histologic characteristics, as well as the carcinogenesis of the disease of interest, to enable high-fidelity experimentation and impactful translational development. In high-grade serous carcinoma of ovarian, tubal, or peritoneal origin, there is a need to regularly re-evaluate the experimental models being employed to address research questions. This review aims to provide a resource for better understanding the applications, strengths, and limitations of the available models. This work discusses the origin and carcinogenesis of high-grade serous carcinoma and reviews normal cell-of-origin models, as understanding the cellular origin is critical to experimental efforts focused on pathogenesis, disease progression, and prevention. High-grade serous carcinoma cell lines are discussed, including essential and complementary molecular features, cell line management, and limitations. Next, the review examines advances in organoids, with a specific focus on patient-derived organoids from ascites and single-cell suspensions, organ-on-a-chip platforms, and tissue slice technologies. Finally, given the heterogeneity of epithelial ovarian cancer in general, and high-grade serous carcinoma in particular, and the evolving understanding of the importance of the tumor microenvironment, in vivo models capture organism-level complexity. In this review, we focused on high-grade serous carcinoma. In this context, we review patient-derive...