Integrated protein and transcriptome spatial profiling

作者：N. Ben-Chetrit, Xiang Niu, Ariel D. Swett, Jesus Sotelo, Maria S Jiao, Caitlin M. Stewart, Catherine Potenski, Paulius Mielinis, Patrick Roelli, Marlon Stoeckius, D. Landau · 发表于：Nature Biotechnology · 年份：2023 · DOI：10.1038/s41587-022-01536-3 · 被引用次数：165 · 研究领域：Medicine

Visium spatial transcriptomics is combined with markers for more than 30 proteins. Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions.