The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection

作者：M. Rao, Zhao Zijun, Yu Lixia, C. Jian, F. Min, Zhang Jie, Li Ming, Weisheng Cao · 发表于：Archives of Virology · 年份：2017 · DOI：10.1007/s00705-017-3563-2 · 被引用次数：11 · 研究领域：Biology、Medicine

A novel avian leukosis viruses (ALV) subgroup named ALV-K was recently isolated from Chinese indigenous chickens which is different from the subgroups (A to E and J) that have previously been reported to infect chickens. More and more ALV-K strains have recently been isolated from local breeds of Chinese chickens. However, there are no more effective diagnostic methods for ALV-K other than virus isolation followed by envelope gene sequencing and comparison. Viral infection can be blocked through expression of the viral receptor-binding protein. In this study, we have engineered a cell line, DF-1/K, that expresses ALV-K env protein and thereby confers resistance to ALV-K infection. DF-1/K can be used in combination with the ALV-K susceptible cell line DF-1 as a specific diagnostic tool for ALV-K and provides a good tool for further research into the molecular mechanisms of interaction between ALV-K env protein and the host cell receptor.