The first Tissue OMIP is out and alternative modalities to analyze single cells and particles

作者：A. Tárnok · 发表于：Cytometry Part A · 年份：2023 · DOI：10.1002/cyto.a.24724 · 研究领域：Medicine

This issue introduces an innovation. So far, optimized phenotyping protocols (OMIPs) were strictly connected to the analysis of technology flow cytometry, may it be fluorescence or metal isotope based. So, if tissues were the biological material of interest, they needed to be prepared by enzymatic and mechanical means to obtain single-cell suspensions. This biological material enabled fast rapid and highcontent analysis of individual cells but sacrificed their contextuality. In addition, it was unknown to what extent the tissue preparation affected the results because fragile and vulnerable cells can get preferentially destroyed. But there was often no other way to handle tissue material for quantitative high-content analysis and many OMIPs (our OMIPs) have been published using tissue-derived single-cell suspensions. This has changed now. In this March issue of Cytometry Part A, you can study the first ever published Tissue OMIP. It is authored by Birrer, Brodie and Stroka (OMIP 88; pp.189–192). The OMIP reports a standardized way to study individual blood-borne and tissue-derived cells in murine liver tissue specimens. In addition, the assay was also tested for murine lung, colon, and spleen tissue. For single cell identification, 20 different metal-isotope-tagged monoclonal antibodies were used for labeling phenotypic and functional markers. The tissue was then subsequently analyzed using an imaging mass cytometer. As you might imagine, the requirements for a Tissue OMIP di...