The potential roles of IL-6 and NKG2C in the pathogenesis of Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis

作者：El-Khateeb Hanin *, S. Malika, I. O, D. A, Campbell E, B. P, Mudaliar K, Speiser J, B. C · 发表于：Clinical Research and Clinical Reports · 年份：2024 · DOI：10.31579/2835-8325/071

Stevens Johnson Syndrome/Toxic Epidermal Necrolysis (SJS/TEN) are most often drug-induced severe cutaneous adverse reactions with increased morbidity and mortality. Eyes are often involved causing ocular surface disease leading to visual impairment and blindness. The pathogenesis is not completely understood. HLA-E-specific activating receptor CD94/NKG2C are reported to trigger T-cell receptor-independent cytotoxicity in cytotoxic T cells. Several cytokines are known to play a role in the pathogenesis of this condition. The role of IL-6 and NKG2C in causing ocular surface disease and keratinocyte apoptosis is not fully explored. Determination of the expression of IL-6 and NKG2C studied by immunofluorescence microscopy will enable better understanding of their potential roles in the pathogenesis of SJS/TEN. It is hypothesized that IL-6 and NKG2C play a crucial role in the pathogenesis of SJS/TEN and may correlate with the degree of severity of skin detachment and ocular surface disease. To validate this hypothesis, the specific aim is to determine the expression of IL-6 and NKG2C in the skin of patients with biopsy confirmed SJS/TEN compared to the lichen planus controls by immunohistochemistry and correlation with severity of skin detachment. Under a current, Loyola IRB approved protocol, 12 collected and archived unstained slides of skin and blood plasma samples from patients with biopsy confirmed SJS/TEN were used for this study. Deconvolution Immunofluorescence (IF) was pe...