Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assay

作者：Eleni P. Mimitou, A. Cheng, A. Montalbano, S. Hao, Marlon Stoeckius, Mateusz Legut, T. Roush, A. Herrera, Efthymia Papalexi, Z. Ouyang, R. Satija, Neville E. Sanjana, Sergei B Koralov, Peter Smibert · 发表于：Nature Methods · 年份：2019 · DOI：10.1038/s41592-019-0392-0 · 被引用次数：442 · 研究领域：Biology、Medicine

Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples. ECCITE-seq combines the single-cell analysis of multiple modalities, for example transcriptome, immune cell receptors, cell surface proteins and single-guide RNAs.