Quercetin increases the antidepressant‐like effects of sclareol and antagonizes diazepam in thiopental sodium‐induced sleeping mice: A possible GABAergic transmission intervention

作者：Mehedi Hasan Bappi, Md Nayem Mia, S. Ansari, Irfan Aamer Ansari, Abdullah Al Shamsh Prottay, M. Akbor, H. El-Nashar, Mohamed El-Shazly, Mohammad S. Mubarak, Muhammad Torequl Islam · 发表于：Phytotherapy Research · 年份：2024 · DOI：10.1002/ptr.8139 · 被引用次数：29 · 研究领域：Medicine

Quercetin is the most common polyphenolic flavonoid present in fruits and vegetables demonstrating versatile health‐promoting effects. This study aimed to examine the effects of quercetin (QR) and sclareol (SCL) on the thiopental sodium (TS)‐induced sleeping and forced swimming test (FST) mouse models. SCL (1, 5, and 10 mg/kg, p.o.) or QR (50 mg/kg, p.o.) and/or diazepam (DZP) (3 mg/kg, i.p.) were employed. After 30 min of TS induction, individual or combined effects on the animals were checked. In the FST test, the animals were subjected to forced swimming after 30 min of administration of the test and/or controls for 5 min. In this case, immobility time was measured. In silico studies were conducted to evaluate the involvement of GABA receptors. SCL (5 and 10 mg/kg) significantly increased the latency and decreased sleeping time compared to the control in the TS‐induced sleeping time study. DZP (3 mg/kg) showed a sedative‐like effect in animals in both sleeping and FST studies. QR (50 mg/kg) exhibited a similar pattern of activity as SCL. However, its effects were more prominent than those of SCL groups. SCL (10 mg/kg) altered the DZP‐3‐mediated effects. SCL‐10 co‐treated with QR‐50 significantly (p < 0.05) increased the latency and decreased sleep time and immobility time, suggesting possible synergistic antidepressant‐like effects. In silico studies revealed that SCL and QR demonstrated better binding affinities with GABAA receptor, especially α2, α3, and α5 subunits. Bot...