Western Blot

作者：H. P. Aslim, Oya Bulut · 发表于：Journal of Advances in VetBio Science and Techniques · 年份：2019 · DOI：10.1007/978-3-662-48986-4_3674 · 被引用次数：194

Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Binding of the antibody is detected using a radioactive or chemical tag. A western blot is sometimes used to diagnose disease.