The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos

作者：Kyungyong Seong, Rakesh Kumar, D. Prigozhin, C. Lunde, Thales Henrique Cherubino Ribeiro, Sébastien Bélanger, Jo-Wei Allison Hsieh, McCree Tang, Blake C. Meyers, K. Krasileva · 发表于：New Phytologist · 年份：2026 · DOI：10.1111/nph.71006 · 研究领域：Medicine

Triticum turgidum cv Kronos is a tetraploid wheat cultivar that underpins one of the most widely used community platforms for functional genomics. Over the past decade, researchers have generated c. 3000 exome‐capture (EC) and promoter‐capture (PC) datasets linked to mutagenized seed stocks, along with extensive transcriptomic and phenotypic resources. However, the absence of a reference genome has constrained their full utility. We assembled a chromosome‐scale reference genome for Kronos, with high‐confidence annotations, including manual curation of over 1000 disease resistance (nucleotide‐binding leucine‐rich repeat (NLR)) genes and genome‐wide identification of microRNAs and phasiRNAs. We additionally reanalyzed EC and PC data to capture mutational landscapes across ethyl methane sulphonate‐mutagenized Kronos populations. We revealed previously hidden NLR diversity and resolved their genomic organization at chromosomal ends. Re‐analysis of capture datasets enabled high‐resolution mutation discovery in genes and regulatory regions, providing a more comprehensive view of the variations detectable in the Kronos mutant populations. Collectively, these resources provide a reference‐quality genomic framework for Kronos and position it as a versatile platform for functional and translational wheat research.