GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells

作者：D. Stephenson, G. Keele, Ariel M Hay, Monika Dzieciatkowska, J. Reisz, Zachary B. Haiman, Amy L Moore, T. Nemkov, Xutao Deng, M. Stone, Kirk C. Hansen, Steven Kleinman, P. Norris, Michael P. Busch, Gary A. Churchill, Brent R Stockwell, N. Roubinian, James C Zimring, Grier P. Page, Angelo D’Alessandro · 发表于：Blood. Red cells & iron · 年份：2025 · DOI：10.1016/j.brci.2025.100020 · 被引用次数：11 · 研究领域：Medicine

Red blood cell (RBC) membrane lipid peroxidation during blood bank storage profoundly impacts transfusion efficacy; however, the genetic determinants underlying RBC resilience remain incompletely defined. Here, we identify a critical role for glutathione peroxidase 4 (GPX4) - a pivotal enzyme protecting against iron-dependent lipid peroxidation (ferroptosis) - in regulating RBC storage quality and post-transfusion survival. Conditional erythroid-specific deletion of Gpx4 in mice exacerbated lipid hydroperoxide accumulation, oxidation and ubiquitination of membrane proteins, and reduced RBC recovery after transfusion. Multi-omics analyses in 13,091 human blood donors from the REDS RBC Omics cohort identified regulatory intergenic (rs8178962), intronic and missense genetic variants in GPX4 (rs73507255, rs8178967), particularly prevalent among donors of African descent, that were linked to increased lipid peroxidation and compromised post-transfusion hemoglobin increments. Single protein- and metabolome-wide association studies (pQTL/mQTL) highlighted genetic variants associated with enhanced (rs8178962) or impaired GPX4 expression, disrupted glutathione homeostasis, lipid hydroperoxide accumulation, accelerated membrane damage, and activation of ferroptotic signatures during RBC storage. These effects were exacerbated by genetic traits impairing redox homeostasis, including glucose 6-phosphate dehydrogenase (G6PD) deficiency (African variant rs1050828 V68M/N126D). Storage of mu...