Community reconfiguration in hydrogen-driven denitrification under oxidized co-contaminants: Shift or shuffle.

作者：Zihang Gao, Yichang Shen, Wenyu Zhang, Yixin Jian, Haixiang Li, Leiyu Feng, Wei-Qin Zhuang, Lijie Zhou · 发表于：Bioresource Technology · 年份：2026 · DOI：10.1016/j.biortech.2026.135452 · 研究领域：Medicine

Low-carbon nitrogen removal requires H2-driven microbiomes that remain stable under oxidized co-contaminant stress. We compared hydrogenotrophic communities exposed to Cr(VI), Se(VI), and As(V) + perfluorooctanoic acid (PFOA) in membrane biofilm reactors (MBfRs) and sequencing batch reactors (SBRs), using 16S rRNA profiling and compositional data analysis. Cr(VI) and Se(VI) served as single-inorganic-oxyanion references, while As(V) + PFOA represented a complex co-stress scenario to test backbone persistence under dual toxicity. We defined Shift as directional taxonomic turnover and Shuffle as abundance reweighting within a conserved core. Although α-diversity and genus-level dominance varied with dose, neither Bray-Curtis nor Aitchison ordination showed significant global separation among stressors (permutational multivariate analysis of variance, PERMANOVA: Bray-Curtis R2 = 0.201, p = 0.356; Aitchison R2 = 0.182, p = 0.796; Permutational analysis of multivariate dispersion, PERMDISP: p > 0.05 for both), a pattern consistent with shuffle-dominant change rather than wholesale community replacement. Set-overlap and Sankey analyses revealed conserved family- and phylum-level backbones centered on Comamonadaceae, Rhodocyclaceae, and Proteobacteria-centered routes. An integrated Stability-Ubiquity-Abundance (SUA) scoring framework with leave-one-sample-out sensitivity analysis prioritized ten candidate cross-contaminant generalists, including Pseudomonas, Stenotrophomonas, Azospi...