Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP–DAPA) System

作者：M. A. Carpenter · 年份：2022 · DOI：10.1021/acschembio.0c00419.s002

The development and function of tissues, blood, and the immune system is dependent upon proximity for cellular recognition and communication. However, the detection of cell-to-cell contacts is limited due to a lack of reversible, quantitative probes that can function at these dynamic sites of irregular geometry. Described here is a novel chemo-genetic tool developed for Corresponding Author Marcel P. Bruchez – Carnegie Mellon University, Department of Chemistry, Department of Biological Sciences, and Molecular Biosensor and Imaging Center, Pittsburgh, Pennsylvania 15213, United States; bruchez@cmu.edu. ∥Author Contributions First authors contributed equally Supporting Information The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acschembio.0c00419. Detailed methods, descriptions for all materials and measurements, comprehensive synthetic details and analytical results for all prepared molecules, descriptions of plasmid DNA prepared, and associated Addgene ID for deposited plasmids; extended biochemical data for the various constructs characterized and reported in summary in the main figures; additional imaging and cytometry data supporting the function of prepared plasmids, image data showing a variety of cell-contact experiments, and observations of varied contact behavior using the FAP–DAPA system included as annotated still images (PDF) Time-lapse video showing dynamic cell contacts visualized over an hour in confocal microscopy (MP...