HS-GC–MS analysis of volatile organic compounds after hyperoxia-induced oxidative stress: a validation study

作者：T. Lilien, Dominic W. Fenn, P. Brinkman, L. Hagens, M. Smit, N. Heijnen, J. V. van Woensel, Lieuwe D. J. Bos, R. Bem, Cristian Nicolaie Presura · 发表于：Intensive Care Medicine Experimental · 年份：2024 · DOI：10.1186/s40635-024-00600-3 · 被引用次数：5 · 研究领域：Medicine

Exhaled volatile organic compounds (VOCs), particularly hydrocarbons from oxidative stress-induced lipid peroxidation, are associated with hyperoxia exposure. However, important heterogeneity amongst identified VOCs and concerns about their precise pathophysiological origins warrant translational studies assessing their validity as a marker of hyperoxia-induced oxidative stress. Therefore, this study sought to examine changes in VOCs previously associated with the oxidative stress response in hyperoxia-exposed lung epithelial cells. A549 alveolar epithelial cells were exposed to hyperoxia for 24 h, or to room air as normoxia controls, or hydrogen peroxide as oxidative-stress positive controls. VOCs were sampled from the headspace, analysed by gas chromatography coupled with mass spectrometry and compared by targeted and untargeted analyses. A secondary analysis of breath samples from a large cohort of critically ill adult patients assessed the association of identified VOCs with clinical oxygen exposure. Following cellular hyperoxia exposure, none of the targeted VOCs, previously proposed as breath markers of oxidative stress, were increased, and decane was significantly decreased. Untargeted analysis did not reveal novel identifiable hyperoxia-associated VOCs. Within the clinical cohort, three previously proposed breath markers of oxidative stress, hexane, octane, and decane had no real diagnostic value in discriminating patients exposed to hyperoxia. Hyperoxia exposure of a...