Spatial organization of the somatosensory cortex revealed by osmFISH

作者：Simone Codeluppi, Lars E. Borm, Amit Zeisel, Gioele La Manno, J. A. van Lunteren, C. Svensson, S. Linnarsson · 发表于：Nature Methods · 年份：2018 · DOI：10.1038/s41592-018-0175-z · 被引用次数：606 · 研究领域：Biology、Medicine

Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type–specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex. osmFISH applies automated cycles of single-molecule fluorescence in situ hybridization without barcoding to provide spatial gene expression in tissue sections at high sensitivity, accuracy and throughput.