Seq-Well: A Portable, Low-Cost Platform for High-Throughput Single-Cell RNA-Seq of Low-Input Samples

作者：Todd M. Gierahn, M. Wadsworth, T. Hughes, Bryan D. Bryson, Andrew W. Butler, R. Satija, S. Fortune, J. C. Love, A. Shalek · 发表于：Nature Methods · 年份：2017 · DOI：10.1038/nmeth.4179 · 被引用次数：708 · 研究领域：Medicine、Biology、Chemistry

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.