Tracking early mammalian organogenesis – prediction and validation of differentiation trajectories at whole organism scale

作者：Iván Imaz-Rosshandler, C. Rode, C. Guibentif, M. Ton, Parashar Dhapola, Daniel Keitley, R. Argelaguet, F. Calero-Nieto, J. Nichols, J. Marioni, M. D. de Bruijn, B. Göttgens · 发表于：bioRxiv · 年份：2023 · DOI：10.1101/2023.03.17.532833 · 被引用次数：28 · 研究领域：Biology、Medicine

Early organogenesis represents a key step in animal development, where pluripotent cells divide and diversify to initiate formation of all major organs. Here, we used scRNA-Seq to profile over 300,000 single cell transcriptomes sampled in 6 hour intervals from mouse embryos between E8.5 and E9.5. Combining this dataset with our previous E6.5 to E8.5 atlas resulted in a densely sampled time course of over 400,000 cells from early gastrulation to organogenesis. Computational lineage reconstruction at full organismal scale identified complex waves of blood and endothelial development, including a new molecular programme for somite-derived endothelium. To assess developmental fates across the primitive streak, we dissected the E7.5 primitive streak into four adjacent regions, performed scRNA- Seq and predicted cell fates computationally. We next defined early developmental state/fate relationships experimentally by a combination of orthotopic grafting, microscopic analysis of graft contribution as well as scRNA-Seq to transcriptionally determine cell fates of the grafted primitive streak regions after 24h of in vitro embryo culture. Experimentally determined fate outcomes were in good agreement with the fates predicted computationally, thus demonstrating how classical grafting experiments can be revisited to establish high-resolution cell state/fate relationships. Such interdisciplinary approaches will benefit future studies in both developmental biology as well as guide the in v...