Nanostructural and mechanical changes in the sclera following proteoglycan depletion

作者：Zhuola Zhuola, S. Barrett, Y. A. Kharaz, R. Akhtar · 发表于：Modeling and Artificial Intelligence in Ophthalmology · 年份：2018 · DOI：10.35119/maio.v2i2.65 · 被引用次数：5 · 研究领域：Chemistry

The mechanical properties of ocular tissues, such as the sclera, have a major impact on healthy eye function, and are governed by the properties and composition of the microstructural components. For example, biomechanical degradation associated with myopia occurs alongside a reduction of proteoglycans (PGs). In this study, the role of PG degradation in the nanomechanical properties of the porcine sclera is explored. In-vitro enzymatic degradation of PGs was conducted with α-amylase and chondroitinase ABC enzymes. Collagen fibril morphology and nanomechanical stiffness were measured with atomic force microscopy (AFM). The elastic modulus of the tissue was reduced in all enzyme-treated samples relative to controls. In addition, collagen fibril organization was disrupted by PG depletion. Our data demonstrate that PGs play an important role in determining not only the mechanical properties at these length scales, but also collagen fibril arrangement.