Anticipating and identifying collateral damage in genome editing

作者：G. Burgio, L. Teboul · 发表于：Trends in Genetics · 年份：2020 · DOI：10.1016/j.tig.2020.09.011 · 被引用次数：41 · 研究领域：Medicine、Biology

Genome editing has powerful applications in research, health care and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on and away from the target locus. This has considerable impact in providing a safe approach for therapeutic gene editing, agriculture and other applications. This Opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.