Quercetin Alleviates Kidney Damage Caused by Mercury Chloride：The protective effects of quercetin on autophagy and inflammation were studied based on TRIM32/TLR4/LC3 pathway.

作者：Fan Jin, Jiayue Li, Chenyu Zhao, Lixiang Gu, Min Pu, Simin Jiang, Mingming Liang, Ying Zhao, Jian-Ren Shen, Agabuwei, Qin Han, Dan Liao · 发表于：Toxicon · 年份：2024 · DOI：10.1016/j.toxicon.2024.108031 · 被引用次数：8 · 研究领域：Medicine

OBJECTIVE Mercury is one of the heavy metal pollutants causing serious harm to human health. Quercetin was observed to repair kidney damage through the TLR4/TRIM32 pathway, and the detoxification effect of quercetin on heavy metal poisoning was observed. METHODS For the study, the researchers divided 40 male mice from the KM strain into five groups: control, HgCl2, QU30, HgCl2+QU15, and HgCl2+QU30. The biological effects of those mice in each group were detected by the biochemical experiment, histopathology experiment and protein expression experiment respectively. RESULTS HgCl2 had effects in increasing the level of malondialdehyde (MDA) and decreasing the activity of antioxidant enzymes (P<0.05). HgCl2 induced inflammation by increasing tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and Toll Like Receptor 4 (TLR-4) (P< 0.05). The expression of creatinine (CRE) and urea nitrogen (BUN) showed that HgCl2 promoted kidney injury. HgCl2 altered renal tissue integrity and TRIM32 expression which resulted in the increased autophagy associated protein levels of LC3. In contrast, quercetin reduced oxidative stress, autophagy, inflammation and histopathological changes (P< 0.05). CONCLUSION Quercetin has the renal protection effects of anti-inflammation, anti-oxidation and anti-autophagy.