Brief guide to western blot assays

作者：Seokjun G. Ha, Ji-Hoon Park, Mi-Young Kim, Seung-Jae V. Lee · 发表于：Molecules and Cells · 年份：2025 · DOI：10.1016/j.mocell.2025.100297 · 被引用次数：18 · 研究领域：Medicine

Western blot is an essential method that detects specific proteins using antibodies, which is one of the most widely applied techniques for protein detection. Here, we present a brief guide to western blot, following the overall procedure from the choice of antibodies to quantification. This guide will provide useful information for researchers who are unfamiliar with western blot assays.