[Improving effect and mechanism of Bugan Decoction on sperm quality in mice with exercise-induced fatigue via PARP-1]

作者：Hu X, Xiao M, Jiang XC, Tang KY, Liu JS, Zou Y, Yu XM, Fang ZP · 发表于：Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica · 年份：2026 · DOI：10.19540/j.cnki.cjcmm.20260206.401 · 研究领域：Spermatozoa、Drugs, Chinese Herbal、Fatigue、Poly (ADP-Ribose) Polymerase-1、Animals、Male、Mice、Mice, Inbred ICR、Physical Conditioning, Animal、Spermatogenesis、Sperm Motility、Humans

This study aimed to investigate the effect and mechanism of Bugan Decoction on spermatogenesis in mice with exercise-induced fatigue. Male ICR mice were screened by swimming training and randomly divided into seven groups(n=10 per group): a blank group, a model group, low-, medium-, and high-dose Bugan Decoction groups, an olaparib group, and a vitamin C group. Except for the blank group, the other groups were subjected to weight-loaded swimming training to establish the exercise-induced fatigue model. After weight-loaded swimming, the low-, medium-, and high-dose Bugan Decoction groups were intragastrically administered 0.149, 0.298, and 0.596 g·kg~(-1) Bugan Decoction, respectively; the olaparib group received intraperitoneal injection of 0.05 g·kg~(-1) olaparib; the vitamin C group was intragastrically given 0.2 g·kg~(-1) vitamin C; the blank group and model group were gavaged with an equal volume of normal saline. The administration lasted for 2 consecutive weeks. At the end of the experiment, the final exhaustive swimming time of mice in each group was recorded. Commercial kits were used to detect the levels of urea nitrogen(BUN), lactic acid(LA), lactate dehydrogenase(LDH), superoxide dismutase(SOD), malondialdehyde(MDA), glutathione peroxidase(GSH-Px) in serum, and testicular testosterone(T), interleukin-6(IL-6), interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) in testes. Pathological morphological changes of muscle and testicular tissues, as well as epididymal sp...