Insights into the conversion of odor compounds by a novel microbial agent during swine manure storage: a multi-omics integration of microbiome, metabolome and genome

作者：Su W, Yi Q, Du M, Gong T, Yang M, Wang F, Jin M, Wang Y, Lu Z · 发表于：Waste management (New York, N.Y.) · 年份：2026 · DOI：10.1016/j.wasman.2026.115724 · 研究领域：Deep-pit swine manure storage、Functional genes、Microbial agent、Microbial communities、Odorous compounds

Deep-pit manure storage in intensive swine farming has emerged as a major source of malodorous emissions, posing significant environmental and public health concerns. This study developed a targeted microbial agent by systematic screening of malodor-degrading microorganisms (Alcaligenes faecalis A1, Bacillus velezensis B6, Pediococcus pentosaceus L4 and Meyerozyma guilliermondii Y7), and multi-omics approach was used to elucidate the mechanism of odorous compounds conversion during manure storage after inoculation with microbial agent. The agent significantly reduced emissions of NH3 (42.22%) and H2S (48.90%), concurrently decreasing key malodorous compounds (phenol, 4-ethylphenol, 3-methylindole, methyl mercaptan, putrescine). LC-MS revealed the dynamic changes of these major malodor substances, their precursors and metabolites during the treatment of microbial agent. High-throughput sequencing identified core functional taxa driving odor mitigation, including bacteria (Alcaligenes, Bacillus, Pediococcus, Rhodopseudomonas, Lysinibacillus and Sedimentibacter) and fungi Meyerozyma, Kurtzmaniella, Mucor, Rhizopus and Candida). Network correlation analysis revealed that the inoculated microbial agent was negatively associated with odor-producing microbiota, while positively associated with microbiota potentially involved in odor abatement, with the inoculated strain Alcaligenes faecalis A1 playing a particularly prominent role. Whole-genome sequencing highlighted abundant odor-m...