Carbapenemase-producing hypervirulent Klebsiella pneumoniae in Alberta, Canada, 2016-2024

作者：Edirmanasinghe R, Dingle TC, Croxen MA, Boyd DA, Mataseje L · 发表于：Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 年份：2026 · DOI：10.3138/jammi-2025-0028 · 被引用次数：56 · 研究领域：Klebsiella sp、antimicrobial resistance、bacterial infections、carbapenemase-producing Enterobacteriaceae

BACKGROUND: Hypervirulent Klebsiella pneumoniae (hvKp) can cause invasive community-acquired infections in healthy adults. HvKp is usually susceptible to most antibiotics; however, multi-drug resistance, including to carbapenems, is emerging. This is especially concerning due to the combination of disease severity and antibiotic resistance. Recent reports of the ST23 lineage of hvKp has raised alarms globally, making reporting of these cases significant. METHODS: Carbapenemase-producing K. pneumoniae (CP-Kp) in Alberta, Canada were screened for the presence of hypervirulence genes associated with the hypervirulent phenotype. A hypermucoviscous string test was performed on all isolates, and genomes were sequenced using both Illumina NextSeq and Oxford Nanopore Technologies platforms. The presence of hypervirulence-associated genes (ybt, clb, iuc, iro, rmpA/A2) in the assembled genomes was determined. RESULTS: Eight different sequence types (ST) were observed among the 16 cases identified, including several global high-risk lineages (ST11, ST15, ST231, ST147, and ST395) and one hypervirulent lineage (ST23). Carbapenemase genes included bla NDM-1 (n = 1), bla OXA-48-type (n = 7), or both bla NDM + bla OXA-48-type (n = 8). The ST23 isolate harboured ybt and clb on the chromosome, iro and iuc on a 229.9 kb IncFIB,IncHI1B virulence plasmid, and bla OXA-48 on a 92.3 kb IncFIA,IncFII plasmid. Almost half of the isolates co-harboured virulence genes and a carbapenemase (bla NDM-1, n...