In-vitro determination of ciprofloxacin - metronidazole interaction by derivative spectrophotometry with greenness profiling

作者：Naeem S, Gul W · 发表于：Pakistan journal of pharmaceutical sciences · 年份：2026 · DOI：10.36721/pjps.2026.39.5.reg.15033.1 · 研究领域：Ciprofloxacin、Metronidazole、Anti-Bacterial Agents、Spectrophotometry, Ultraviolet、Drug Interactions、Spectrophotometry、Hydrogen-Ion Concentration

BACKGROUND: The study of DDIs has evolved significantly over the years, early in-vitro detections could help identify potential interactions before clinical use, enhancing drug efficacy and patient safety. OBJECTIVES: This work therefore focused on finding in-vitro DDI (incompatibility) between the two commonly prescribed antibiotics Ciprofloxacin (CFX) and Metronidazole (MTZ) in neutral, acidic and basic mediums using derivative spectroscopy. METHODS: The spectral measurements were performed on UV-Visible spectrophotometer at 0, 4 and 24 hours. Standard solutions (100ppm) and working solutions (3-11ppm) of both drugs were prepared in water, while mixture contained 5ppm of each drug. AGREE calculator software was used to figure out the environmental impact of the used spectrophotometric method. A zero-crossing method was employed for simultaneous quantification of CFX and MTZ in admixture. RESULTS: It was observed that a mixture having 5ppm CFX and MTZ showed a recovery percentage of 87.704% ± 0.001041 and 78.226% ± 0.002379 respectively in neutral medium at 4 hours, along with AGREE score of 0.8. The proposed method was greener (eco-friendly). CONCLUSION: The findings concluded that although the two drugs were compatible in acidic and basic mediums, these might be incompatible in neutral environment upon simultaneous administration.