In vitro characterization of probiotic properties of isolated and grown co-cultures on halophyte-based substrate

作者：Rudnyckyj S, Zhytniakivska O, Thomsen MH, Čukajne T, Matijašić BB, Lorbeg PM · 发表于：International microbiology : the official journal of the Spanish Society for Microbiology · 年份：2026 · DOI：10.1007/s10123-026-00786-4 · 被引用次数：125 · 研究领域：Probiotics、Salt-Tolerant Plants、Lactobacillales、Yeasts、Sodium Chloride、Coculture Techniques、Antioxidants、Fermentation、Microbial Viability

Probiotics are increasingly recognized for their health-promoting effects, yet their performance in unconventional fermentation systems such as halophyte-based substrates remains poorly understood. Halophytes, salt-tolerant plants rich in phenolics and other bioactive compounds, impose selective pressures that may favor robust and stress-tolerant microorganisms. In this study, we assessed the probiotic potential of selected lactic acid bacteria and yeast strains, including Lactococcus (L.) lactis, Bacillus (B.) coagulans ATCC 7050, Saccharomyces (S.) cerevisiae, Kluyveromyces (K.) marxianus DSM 7238, and Cyberlindnera (C.) jadinii DSM 2361 and DSM 70,163, cultivated on halophyte hydrolysates. In addition, newly isolated strains from halophyte biomass, Lactiplantibacillus (Lpl.) plantarum, Levilactobacillus (Lev.) brevis, and Pichia (P.) fermentans, were bioprospected for their probiotic potential and robustness against relevant environmental stressors. Strains were assessed for viability, tolerance to NaCl and phenol, survival under simulated gastrointestinal conditions, cell surface properties (hydrophobicity, autoaggregation, biofilm formation), antioxidant activity, and antimicrobial effects. Results revealed great variability among tested strains. Lpl. plantarum and L. lactis demonstrated high salt (> 70% survivability at 5% NaCl) and phenol tolerance (> 80% survivability at 0.3% phenol), strong survivability in simulated digestion (up to ~ 30% survival for Lpl. plantarum...