Generation of chimeric chickens resistant to infection by avian leukemia virus subgroups A, B, D, E, and K through primordial germ cells

作者：Xiao G, Chen W, Chen X, Huang Y, Pan T, Chen S, Wen Y, Li H, Zhang X, Xie Q · 发表于：Poultry science · 年份：2026 · DOI：10.1016/j.psj.2026.106642 · 被引用次数：33 · 研究领域：Chickens、Avian Leukosis Virus、Avian Leukosis、Germ Cells、Chimera、Disease Resistance、Poultry Diseases、Animals、Female、Male、Chick Embryo

Avian leukosis virus (ALVs), an oncogenic retrovirus, induces immunosuppression and various tumors in chickens, posing a severe global threat to poultry health and causing substantial economic losses. Natural resistance alleles, notably tvar2 and tvbr3, confer genetic resistance to specific ALV subgroups (ALV-A, -K, and ALV-B, -D, -E, respectively). This study aimed to exploit this resistance by creating resistant chicken lines via primordial germ cells (PGCs)-mediated chimera. We first genotyped the tvar2 and tvbr3 loci in Qingyuan Partridge Chickens (QYPCs) and Zhanjiang Yellow Chickens (ZJYCs). Through selective mating, we generated homozygous tvar2/r2 and tvbr3/r3 QYPC embryos as PGC donors, and homozygous susceptible tvas/s and tvbs/s ZJYC embryos as recipients. Donor PGCs harvested from QYPC embryos were successfully cultured in vitro, as confirmed by the expression of germ cell-specific markers (DDX4, c-Kit, PRDM14) and scanning electron microscopy revealing characteristic microvilli. These PGCs were microinjected into recipient embryos, producing viable chimeric chickens. Genotyping confirmed the successful integration of the resistant donor-derived PGCs. The germline transmission efficiency reached 35.7%. Our findings demonstrate that PGC-mediated germline transmission provides an efficient strategy for rapidly establishing ALV-resistant poultry flocks, offering a potential strategy for establishing ALV-resistant poultry flocks by introgressing resistance alleles.