In vitro growth of secondary follicles from cryopreserved-thawed ovarian cortex

作者：Cheng H, Wei F, Del Valle JS, Stolk THR, Huirne JA, Asseler JD, Pilgram GSK, Van Der Westerlaken LAJ, Van Mello NM, Chuva De Sousa Lopes SM · 发表于：Human reproduction (Oxford, England) · 年份：2024 · DOI：10.1093/humrep/deae240 · 研究领域：in vitro culture、fertility preservation、follicular growth、granulosa cells、human、oocyte、ovarian cortex、ovarian tissue cryopreservation、secondary follicle、trans masculine

STUDY QUESTION: Can secondary follicles be obtained from cultured cryopreserved-thawed human ovarian cortical tissue? SUMMARY ANSWER: We obtained high-quality secondary follicles from cultured cryopreserved-thawed human ovarian cortical tissue from cis female donors (cOVA), but not from trans masculine donors (tOVA) in the same culture conditions. WHAT IS KNOWN ALREADY: The in vitro growth of oocytes present in unilaminar follicles into metaphase II stage (MII) oocytes has been previously achieved starting from freshly obtained ovarian cortical tissue from adult cis female donors. This involved a multi-step culture protocol and the first step included the transition from unilaminar follicles to multilayered secondary follicles. Given that the ovarian cortex (from both cis female and trans masculine donors) used for fertility preservation is cryopreserved, it is crucial to investigate the potential of unilaminar follicles from cryopreserved-thawed ovarian cortex to grow in culture. STUDY DESIGN, SIZE, DURATION: Cryopreserved-thawed ovarian cortical tissue from adult trans masculine donors (n = 3) and adult cis female donors (n = 3) was used for in vitro culture following the first culture step described in two published culture protocols (7-8 days and 21 days) and compared to freshly isolated ovarian cortex from trans masculine donors (n = 3) and to ovarian cortex prior to culture. PARTICIPANTS/MATERIALS, SETTING, METHODS: Ovarian cortical tissue was obtained from adult tr...