Proteomic Profiling of the Interplay Between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies

作者：Sukumaran A, Geddes-McAlister J · 发表于：Methods in molecular biology (Clifton, N.J.) · 年份：2022 · DOI：10.1007/978-1-0716-2124-0_17 · 研究领域：Bacterial pathogen、Macrophage、Mass spectrometry、Quantitative proteomics

Bottom-up proteomics enables a systems-level analysis of proteins involved in a particular sample set. In this protocol, we describe the workflow to prepare Klebsiella pneumoniae and macrophage cells for co-culture, how to extract and prepare samples for analysis by high-resolution mass spectrometry, and lastly, how to analyze the output data files. This workflow allows for the identification of proteins involved in both the bacterial and host perspective during pathogenesis.