Update on techniques for cryopreservation of human spermatozoa

作者：Huang C, Tang YL, Hu JL, Zhou WJ, Huang ZH, Luo XF, Li Z, Zhu WB · 发表于：Asian journal of andrology · 年份：2022 · DOI：10.4103/aja20229 · 研究领域：cryodamage、cryoprotectant、dry preservation、freezing and thawing、sperm cryopreservation

In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.