Discrimination of Irradiated Beef Using Comet Assay

作者：Seok‐Kyu Jeong, Jong‐Heum Park, Seung‐Taek Ji, Kum-Ju Park, Hai-Hong Kim, Chang‐Kee Hyun, Heuyn‐Kil Shin · 发表于：Korean Journal of Food Science and Technology · 年份：2000 · 被引用次数：2 · 研究领域：Radiation Effects and Dosimetry、Agriculture, Soil, Plant Science、Food Quality and Safety Studies

DNA damages in post-mortem bovine muscle samples caused by gamma irradiation at doses of 1 to 10 kGy were determined by Comet assay. When the cell extract was prepared in a physical method and followed by neutral lysis and neutral electrophoresis, the optimal comet images could be obtained. DNA damages were evaluated from the mean tail length, the distributions of comet images in 4 groups divided by tail length and the relative damage index (RDI) values calculated from the distribution pattern. The mean tail length and RDI value were increased by increasing the irradiation dose, and the RDI value was found to be useful as an index for discriminating of irradiation and measuring the irradiated dose. Blind tests using Korean domestic (Hanwoo) and imported beef samples showed a higher RDI value for the latter. However, the value was lower than those of irradiated samples indicating that the cause of DNA damages in the imported beef samples might be not irradiation but low-temperature treatments. It was concluded from the results of this study that the irradiated beef and its irradiated dose could be detected and predicted by Comet assay.