Correction for Plasma Trapped in the Red Cell Column of the Hematocrit

作者：H. Chaplin, Patrick Loudon Mollison · 发表于：Blood · 年份：1952 · DOI：10.1182/blood.v7.12.1227.1227 · 被引用次数：285 · 研究领域：Erythrocyte Function and Pathophysiology、Blood properties and coagulation、Microfluidic and Bio-sensing Technologies

Abstract 1. In the hematocrit, the percentage of the red cell column occupied by trapped plasma increases directly with the packed cell volume; this is due to a corresponding reduction of the effective radius of centrifugation. 2. When P.C.V. is determined in Wintrobe hematocrit tubes spun at 3000 r.p.m. in a centrifuge of radius 15 cm., the variation in plasma trapping between one sample and another is much smaller after 55 minutes centrifugation than after 30 minutes. If a 55 minute period is used, variation is so small, even when pathologic bloods are tested, that it is satisfactory to use a calibration curve. Such a curve is presented.