Supplementary Figure S3 from The Oncogenic <i>EGFR–SHC1</i> Fusion Confers Insensitivity to EGFR-TKIs via Dual Activation of N-EGFR Kinase Domain and C-SHC1 Phosphorylation Sites in Lung Cancer

作者：Jiani Zheng, Shen Zhao, Jianhua Zhan, Weitao Zhuang, Maojian Chen, Wei Jiang, Yihua Huang, Junyi He, Liyang Hu, Lanlan Pang, Feng Hao, Jinhui Xue, Mengting Shi, Anlin Li, Jingxun Wu, Shaodong Hong, Yuanyuan Zhao, Feng Ye, Yan Huang, Hongyun Zhao, Yunpeng Yang, Wenfeng Fang, Li Zhang, Jing Li · 年份：2026 · DOI：10.1158/2159-8290.33143426 · 研究领域：Cell biology、Cancer research、Biology、Chemistry、Molecular biology

<p>Supplementary Figure S3 shows that both the N-terminal EGFR kinase domain and the C-terminal SHC1 phosphorylation sites contribute to the oncogenic properties of EGFR-SHC1, including analyses of SHC1 phosphorylation in response to EGF stimulation, TKI sensitivity in Ba/F3 cells, and functional assessments via soft agar colony formation and IL-3-independent proliferation assays using various EGFR-SHC1 mutants. It also includes immunoblotting validation of protein expression and downstream signaling in cells expressing EGFR-RAD51 and EGFR-SHC1 variants.</p>