Unravelling the regulatory network and evolutionary aspects of the ARID gene family of Arabidopsis through in- silico analyses

作者：Pinki Debnath, Md. Redwan Ahmed, Nahida Akter, Rounak Jahan Raka, Kakon Chakma · 发表于：Discover Plants. · 年份：2026 · DOI：10.1007/s44372-026-00775-x · 研究领域：Chromatin Remodeling and Cancer、RNA regulation and disease、Biotin and Related Studies

Abstract The AT-rich interaction domain ( ARID ) gene family is a conserved group of DNA- binding proteins involved in chromatin remodeling, transcriptional regulation, and eukaryotic developmental processes. Despite having functional importance, no comprehensive genome-wide study has been conducted on this gene family. In this study, genome-wide analysis was conducted through an in-silico approach on the model plant Arabidopsis thaliana to characterize the ARID gene family. A total of ten AtARID genes were identified and systematically analyzed for their chromosomal localization, gene structure, gene ontology, conserved motifs, cis-regulatory elements, phylogenetic relationships, and expression profiles. The predicted AtARID proteins vary widely in molecular weight, isoelectric point, and exon–intron organization, indicating structural and functional diversification. All identified genes contained the ARID domain and were localized to chromosomes 1–4, with subcellular localization in the nucleus. Analysis revealed the presence of core cis-elements (TATA- and CAAT-box) and multiple hormone and stress-responsive motifs, including ABRE and W-box, implying complex transcriptional regulation. Phylogenetic and synteny analyses across Arabidopsis thaliana , rice, maize, wheat, citrus, and tomato revealed five major clades while several segmental duplication events were identified within Arabidopsis thaliana . Transcriptomic profiling showed tissue-specific expression patterns, with...