Microbial single-cell sequencing technologies: Principles, applications, and spatial integration

作者：Fei Xu, Fangyu Mo, Babatunde O. Kehinde, Qinghong Qian, Longjiang Fan, Weiqin Jiang, Hongyu Chen · 发表于：Biotechnology Advances · 年份：2026 · DOI：10.1016/j.biotechadv.2026.108996 · 研究领域：Single-cell and spatial transcriptomics、bioluminescence and chemiluminescence research、Cell Image Analysis Techniques

The advent of microbial single-cell sequencing technology provides unprecedented resolution to study microbial ecosystems, revealing cellular heterogeneity, interactions, and genetic evolution of microorganisms. This review systematically compiles current microbial single-cell sequencing technologies, providing a detailed synthesis of their underlying technical principles, illuminating the strengths and limitations of various approaches. In-depth summaries of the technical challenges they encounter in different microbial domains and their practical applications are provided. Finally, we summarized the emerging field of microbial spatial omics, with a particular focus on advanced imaging techniques utilizing sequencing and fluorescence in situ hybridization.