Metagenomic analysis of commensal small mammal samples from an international cargo shipping area, Bangkok Port, reveals potential zoonotic pathogens and implications for One Health surveillance

作者：Dararat Sirimongkol, Peerawat Wongluechai, Tatiyanuch Chamsai, Thekhawet Weluwanarak, Narinthip Chaipromkhieo, Nareerat Sangkachai, Kanittha Tonchiangsai, Choenkwan Pabutta, Pornchai Kerdsiri, Ladawan Sariya · 发表于：One Health · 年份：2026 · DOI：10.1016/j.onehlt.2026.101534 · 研究领域：Zoonotic diseases and public health、Salmonella and Campylobacter epidemiology、Brucella: diagnosis, epidemiology, treatment

Commensal small mammals, such as rats and shrews, are recognized reservoirs for numerous zoonotic pathogens; however, their role in pathogen circulation at transport hubs remains underexplored. This study employs shotgun metagenomic sequencing to characterize microbial communities and assess zoonotic potential in tissue samples from commensal small mammals captured at Bangkok Port, an international cargo shipping hub in Thailand between June and August 2025. Following host read depletion, taxonomic profiling was performed to identify bacterial taxa of public health relevance, including sequences assigned to Bordetella spp., Yersinia pestis , Bartonella elizabethae , and Acinetobacter baumannii . The virulence factor and antibiotic resistance gene profiles revealed that some of these pathogens have pathogenic potential and are related to drug-resistant bacteria. In addition, Y. pestis was identified as a shared taxon among rats, shrews, and their associated fleas. The findings support the ecological roles of mammalian hosts and their ectoparasites as reservoirs for pathogens of public health concern. These results emphasize the need to strengthen surveillance programs for commensal small mammals to monitor and mitigate the spread of transboundary pathogens at international maritime gateways.