Assessment of Ultraviolet-C Inactivation of Pseudomonas aeruginosa and Aspergillus flavus in the Anesthesia Workspace

作者：Randy W. Loftus, Franklin Dexter, Michelle C. Parra, Carmen T. Brindeiro, Chase P. Loftus, Cornelie CL. Banguid, Brendan T. Wanta, Jonathan E. Charnin · 发表于：Mayo Clinic Proceedings Innovations Quality & Outcomes · 年份：2026 · DOI：10.1016/j.mayocpiqo.2026.100740 · 研究领域：Medical Device Sterilization and Disinfection、Infection Control in Healthcare、Nosocomial Infections in ICU

Objective To determine the ultraviolet-C (UV-C) irradiation doses required to inactivate Pseudomonas aeruginosa and Aspergillus flavus contamination on polycarbonate surfaces using a triangular emitter configuration. Patients and Methods In this laboratory experiment, polycarbonate test carriers inoculated with >10 8 colony-forming units of P aeruginosa (MYA-15442) or A flavus (American Type Culture Collection 9643) were positioned 2.74 m from 3 linear UV-C emitters to determine minimally effective doses. Ten independent irradiation doses were evaluated for P aeruginosa (25-250 mJ/cm 2 ) and A flavus (50-2000 mJ/cm 2 ). Larger carriers were additionally evaluated for A flavus to model reduced cell clumping, simulating prior surface disinfection. Residual colony-forming units were quantified and compared with untreated controls to calculate log reductions. Finally, the efficacy of delivering these minimally effective doses through a triangular UV-C emitter configuration was assessed. Results For P aeruginosa , no growth was detected at any dose, including 25 mJ/cm 2 . For A flavus on standard carriers, inactivation occurred at 725 mJ/cm 2 , though no growth conditions were not achieved at any tested dose. However, on larger carriers (reduced clumping), no growth conditions for A flavus were achieved at 650 mJ/cm 2 . Delivery of 25 mJ/cm 2 for P aeruginosa and 650 mJ/cm 2 for A flavus through the triangular configuration resulted in undetectable growth. Conclusion A UV-C dose o...