Effects of Dietary Luteolin on the Growth Performance and Intestinal Health of Juvenile GIFT Tilapia

作者：Xiangli Li, Huige Ren, Jianting Lu, Yonggang Mo, Jingyi Du, Ye Qian, Xiao Peng, Zihe Guo, Chanxia Qin, Chengrui Huang, Kai Huang, Yinghui Zhang, Weihao Ou · 发表于：Antioxidants · 年份：2026 · DOI：10.3390/antiox15080933 · 研究领域：Aquaculture Nutrition and Growth、Animal Nutrition and Physiology、Aquaculture disease management and microbiota

This study aimed to investigate the effects of luteolin on the growth performance and intestinal health of juvenile Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). A total of 450 juvenile GIFT tilapia (initial body weight 8.12 ± 0.04 g) were randomly assigned to five groups (90 fish per group; three replicate tanks of 30 fish each) and fed diets containing luteolin at 0, 50, 150, 300, or 600 mg/kg (CG, LG50, LG150, LG300, and LG600, respectively) for 56 days. The results showed that with increasing luteolin supplementation, the weight gain rate (WGR) and specific growth rate (SGR) first increased and then decreased; the LG300 group had the highest WGR and SGR (significantly higher than the CG and LG600 groups, p < 0.05) and the lowest feed conversion ratio. Based on broken-line and quadratic polynomial regression analyses of the WGR and SGR, the optimal dietary luteolin supplementation level was determined to be 274.14–303.39 mg/kg. For intestinal antioxidant enzyme activities and oxidative stress, total superoxide dismutase (T-SOD) activity in the LG150 and LG300 groups was significantly higher than in the CG and LG600 groups (p < 0.05), and superoxide anion (O2−) content in the LG300 group was significantly lower than in the CG, LG50, and LG600 groups (p < 0.05). Histological analysis of the intestine revealed that muscular layer thickness was significantly greater in the LG300 group than in the CG group (p < 0.05). Intestinal microbiota analy...