Recent advances on bioaffinity fishing technology for discovery of active components from natural products

作者：Qingli QU, Xiaofang WANG, Duolong DI, Pei Dong · 发表于：Chinese Journal of Chromatography · 年份：2026 · DOI：10.3724/sp.j.1123.2025.08021 · 研究领域：Protein Hydrolysis and Bioactive Peptides、Seaweed-derived Bioactive Compounds、Phytochemical compounds biological activities

Natural products have consistently served as an invaluable source for novel drug development， and the efficient and targeted screening of bioactive constituents from these complex mixtures is crucial for advancing drug discovery. Bioaffinity fishing techniques exploit the highly specific interactions between ligands and their biological targets—such as antigen-antibody， enzyme-substrate， and hormone-receptor interactions—to simultaneously separate and identify active compounds from complex natural product extracts. When coupled with liquid chromatography-mass spectrometry， bioaffinity fishing enables the integrated separation， screening， and analysis of bioactive molecules in a single step. This comprehensive review focuses specifically on bioaffinity fishing materials， which are fundamental to the effectiveness and reproducibility of the fishing process. The review systematically explores recent advances over the past three years， categorizing the discussion along two primary dimensions： carrier materials and immobilization methods. In terms of carrier materials， the article provides a comprehensive review of both carrier-free and carrier-based systems. Carrier-free approaches， including affinity ultrafiltration and size exclusion chromatography， utilize free targets in solution， while carrier-based systems immobilize the target onto a solid support. The review details a wide spectrum of innovative supports such as magnetic beads， which facilitate easy separation； metal-orga...