Relationship Between the Proportion of CD24+ CD27+ B Cells And Lung Adenocarcinoma: Evidence From Mendelian Randomization And Single-Cell Analyses

作者：Peiyuan Zhong, Jinhong Chen, Tiantian Song, Li Chen, Zhihui Lu, SuJuan Peng, Xie Zhu, Xinjing Ding, Ping Kong, Jing Wen, Yanqun Zeng, Chunjiao Xiong, Yangyang Liu, Huafeng Liu, Hongxiang Huang · 发表于：Current Medicinal Chemistry · 年份：2026 · DOI：10.2174/0109298673439103260605075257 · 研究领域：Cancer Immunotherapy and Biomarkers、Single-cell and spatial transcriptomics、vaccines and immunoinformatics approaches

BACKGROUND: CD24+ CD27+ B cells play significant roles in multiple diseases. However, the relationship between them and Lung Adenocarcinoma (LUAD) remains elusive. This study is designed to explore the potential causal association between the proportion of CD24+ CD27+ B cells and LUAD, and to deeply understand their role in the pathogenesis of LUAD. METHODS: Genome-Wide Association Study (GWAS) and single-cell RNA sequencing (scRNA-seq) datasets were obtained from online databases. Distinct cell subgroups were annotated using published literature. The Wilcoxon test analyzed cell infiltration proportions, and Differentially Expressed Genes (DEGs) in CD24+ CD27+ B cells between tumor and normal samples were identified. Two-Sample Mendelian Randomization (TSMR) assessed the correlation between CD24+ CD27+ B cell proportions and LUAD, as well as between DEGs and LUAD, treating these as exposure factors and LUAD as the outcome. Sensitivity analysis and MR-Steiger filtering verified results. Cell-cell communication analysis explored interactions between CD24+ CD27+ B cells and other cell types. RESULTS: Nine distinct cell subgroups were identified. CD24+ CD27+ B cell proportions were significantly higher in tumors than in normal samples (P < 0.01). CD24+ CD27+ B cell proportion was a risk factor for LUAD (IVW: OR = 1.388, P = 0.032, 95% CI = 1.029-1.873). DEGs DDX24 (IVW: OR = 1.154, P = 0.013) and SCGB3A1 (IVW: OR = 1.148, P = 0.043) were also identified as risk factors. Cell-cell...