Additional file 4 of Positional identification of a candidate gene for MALE STERILITY 2 (MS2) by linkage mapping and transcriptomic data in Cryptomeria japonica D. Don

作者：Saneyoshi Ueno, Yoichi Hasegawa, Tokuko Ujino‐Ihara, Momi Tsuruta, Hiroyuki Kakui, Junji Iwai, Satoko Hirayama, Katsushi Yamaguchi, Shuji Shigenobu, Takeshi Fujino, Yutaka Suzuki, Masahiro Kasahara, Yoshinari Moriguchi · 发表于：Figshare · 年份：2026 · DOI：10.6084/m9.figshare.32931081 · 研究领域：Plant Reproductive Biology、Plant Taxonomy and Phylogenetics、Chromosomal and Genetic Variations

Supplementary Material 7.Supplementary Figure 3. Temporal expression profile of SUGI_0493010 in male strobili based on the microarray dataset. Description of data: Normalized signal intensities of microarray probes matching SUGI_0493010 are shown across ten developmental stages of male strobili (mean ± SD). Developmental stages are defined according to Tsubomura et al. (2016) as follows: Stage 1, appearance of scale primordia in the axils; Stage 2, differentiation of microsporangia after scale formation; Stage 3, recognizable microsporangial wall, middle layer, tapetum and pollen mother cells; Stage 4, entry of pollen mother cells into meiosis; Stage 5, completion of meiosis and tetrad formation; Stage 6, degeneration of the callose wall and release of microspores into the microsporangium; Stage 7, degeneration of the tapetum; Stage 8, formation of fibrous bands in the microsporangial wall; Stage 9, pollen mitotic division and maturation of pollen grains; Stage 10, anther dehiscence and pollen release. All probes show a pronounced expression peak at intermediate stages (around Stages 5–7), corresponding to the period of tetrad formation, microspore release, and tapetum degeneration.