Additional file 10 of Positional identification of a candidate gene for MALE STERILITY 2 (MS2) by linkage mapping and transcriptomic data in Cryptomeria japonica D. Don

作者：Saneyoshi Ueno, Yoichi Hasegawa, Tokuko Ujino‐Ihara, Momi Tsuruta, Hiroyuki Kakui, Junji Iwai, Satoko Hirayama, Katsushi Yamaguchi, Shuji Shigenobu, Takeshi Fujino, Yutaka Suzuki, Masahiro Kasahara, Yoshinari Moriguchi · 发表于：Figshare · 年份：2026 · DOI：10.6084/m9.figshare.32931102 · 研究领域：Synthesis and bioactivity of alkaloids、Sirtuins and Resveratrol in Medicine、Biological Activity of Diterpenoids and Biflavonoids

Supplementary Material 5.Supplementary Figure 2. Amino-acid sequence alignment of the MS2 mutational candidate genes in Cryptomeria japonica: (A) INTS1 and (B) GELP. Description of data: Alignment of the amino acid sequences of SUGI standard gene set (SUGI_0492850 and SUGI_0493010 for INTS1 and GELP, respectively), Gosenshi-1_a1 (wild-type allele), Gosenshi-1_a2 (mutant allele), and the ms2 mutant from the S1-2 family, generated in CLC Genomics Workbench ver. 20.0.4 (Qiagen). Residues are colored according to side‐chain polarity. Red arrows indicate positions of deleterious amino acid substitutions predicted by PROVEAN (score ≤ –2.5). For INTS1, two deleterious amino acid substitutions (at positions 613 and 1047) were identified in the S1-2 family, but these substitutions were absent in `Gosenshi-1.` For GELP, two substitutions are observed relative to SUGI_0493010 (S40F and C352Y), but only S40F is deleterious and uniquely distinguishes the ms2 mutant from the wild-type allele. In addition, the 352nd residue in `Gosenshi-1` (Ms2/ms2) is homozygous for tyrosine (Y/Y), as indicated by the blue arrow, further indicating that C352Y is unlikely to be the causal mutation.