Additional file 8 of Positional identification of a candidate gene for MALE STERILITY 2 (MS2) by linkage mapping and transcriptomic data in Cryptomeria japonica D. Don
作者:Saneyoshi Ueno, Yoichi Hasegawa, Tokuko Ujino‐Ihara, Momi Tsuruta, Hiroyuki Kakui, Junji Iwai, Satoko Hirayama, Katsushi Yamaguchi, Shuji Shigenobu, Takeshi Fujino, Yutaka Suzuki, Masahiro Kasahara, Yoshinari Moriguchi · 发表于:Figshare · 年份:2026 · DOI:10.6084/m9.figshare.32931096 · 研究领域:Biology、Genetics、Computational biology
Supplementary Material 8.Supplementary Table 5. Genome-wide annotation and expression profiles of GDSL esterase/lipase (GELP) genes in Cryptomeria japonica male strobiliDescription of data: A comprehensive list of 209 GELP candidates is provided together with Pfam-A/HMMER evidence for GDSL esterase/lipase-related domains (including Lipase_GDSL (PF00657.24) and related Pfam entries), and PF00657-specific domtblout-derived coordinates (alifrom–alito) used to extract the PF00657-aligned domain regions for downstream multiple sequence alignment and phylogenetic analysis. PF00657 hits were detected in 191 proteins, and after excluding truncated matches, 162 PF00657 domain sequences were retained for alignment and neighbor-joining tree inference. The table also includes transcript abundance values (TPM) in male strobili for all RNA-Seq samples analyzed in this study, together with the summary column “TPM max. in WT,” which indicates the maximum TPM value among wild-type male strobilus samples. The binary flag “is_GELP_PFAM_any” indicates whether a gene has GDSL-related Pfam support. In the Discussion, the subset referred to as GELP genes with a Pfam GDSL domain and detectable expression in male strobili corresponds to entries with is_GELP_PFAM_any = 1 and TPM max. in WT > 1. Gene-based functional annotations (Araport11 best hits, UniProt and EggNOG information, and predicted protein domains) were extracted from the publicly available ForestGEN annotation dataset for the SUGI_1 r...