Supplemental Figure 8 from Increased Glucose Availability Sensitizes Pancreatic Cancer to Macrophage-Targeting Immunotherapies

作者：Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud, Goutam Dey, Hallie J. Graor, Katie E. Blise, Dove Keith, Shamilene Sivagnanam, Shakti Prasad Pattanayak, Erryk Katayama, Omid Hajihassani, Charles D. Lopez, Rosalie C. Sears, Robert Eil, Lisa M. Coussens, Jonathan R. Brody, Ali Vaziri‐Gohar, Jordan M. Winter · 年份：2026 · DOI：10.1158/2767-9764.32769802 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Cancer Cells and Metastasis

Supplemental Figure 8 - Flow cytometric analysis of additional immune and stromal cell infiltration following single and combination treatments in pancreatic tumors. A.) Representative flow cytometry plots illustrating the effects of vehicle control, D30, PLX3397, PLX3397 + D30, PF-4136309, and PF-4136309 + D30 on stromal fibroblast populations within pancreatic tumors. Tumor-derived single-cell suspensions were first gated on viable cells based on forward- and side-scatter properties. The CD45− fraction was subsequently analyzed to identify CD140α+ fibroblasts, as shown by the indicated gates. Enlarged panels display representative SSC-A versus CD140α plots for each treatment group, with percentages of CD140α+ cells indicated. Bar graphs summarize the proportion of CD45+ immune cells and CD140α+ fibroblasts across treatment groups. B-C.) Representative flow cytometry plots and quantitative analyses showing the impact of vehicle control, D30, PLX3397, PLX3397 + D30, PF-4136309, and PF-4136309 + D30 on intratumoral B cells (B.) and NK1.1+ cells (C.). Representative contour plots display the frequencies of B220+ and NK1.1+ populations within the parent gates, with percentages shown in each panel. Bar graphs summarize the proportion of B220+ B cells and NK1.1+ cells ...